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  Indian J Med Microbiol
 

Figure 2: The restriction digestion pattern of MDR1-C3435T (a), CYP2C19*2-G681A (b), CYP2C19*3-G636A (c) and P2Y12-i-T744C (d) on 1.5% Agarose gel electrophoresis: (a) represents the restriction digestion pattern of MDR1 (C3435T) with Sau3A1.Lane 1: Wild type genotype showing one band of 248bp. Lane 2: Homozygous mutant genotype showing two bands of 208bp and 40bp. Lane 3: Heterozygous genotype showing three bands of 248bp, 208bp and 40bp.(b) represents the restriction digestion pattern of CYP2C19*2 (G681A) with SmaI. Lane 1: Homozygous mutant genotype showing one band of 220bp. Lane 2: Wild type genotype showing two bands of 117bp and 103bp. Lane 3: Heterozygous genotype showing three bands of 220bp, 117bp and 103bp. (c) represents the restriction digestion pattern of CYP2C19*3 (G636A) with BamHI. Lane 1: Homozygous mutant genotype showing one band of 288bp. Lane 2: Wild type genotype showing two bands of 208bp and 122bp. (d) represents the restriction digestion pattern of P2Y12 (i-T744C) with HpyCH4IV.Lane 1: Homozygous mutant genotype showing one band of 266bp. Lane 2: Wild type genotype showing two bands of 175bp and 91bp. Lane M: molecular markers (50 -1500 base pairs)

Figure 2: The restriction digestion pattern of MDR1-C3435T (a), CYP2C19*2-G681A (b), CYP2C19*3-G636A (c) and P2Y12-i-T744C (d) on 1.5% Agarose gel electrophoresis: (a) represents the restriction digestion pattern of MDR1 (C3435T) with Sau3A1.Lane 1: Wild type genotype showing one band of 248bp. Lane 2: Homozygous mutant genotype showing two bands of 208bp and 40bp. Lane 3: Heterozygous genotype showing three bands of 248bp, 208bp and 40bp.(b) represents the restriction digestion pattern of CYP2C19*2 (G681A) with SmaI. Lane 1: Homozygous mutant genotype showing one band of 220bp. Lane 2: Wild type genotype showing two bands of 117bp and 103bp. Lane 3: Heterozygous genotype showing three bands of 220bp, 117bp and 103bp. (c) represents the restriction digestion pattern of CYP2C19*3 (G636A) with BamHI. Lane 1: Homozygous mutant genotype showing one band of 288bp. Lane 2: Wild type genotype showing two bands of 208bp and 122bp. (d) represents the restriction digestion pattern of P2Y12 (i-T744C) with HpyCH4IV.Lane 1: Homozygous mutant genotype showing one band of 266bp. Lane 2: Wild type genotype showing two bands of 175bp and 91bp. Lane M: molecular markers (50 -1500 base pairs)